FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240449848
Sequences flagged as poor quality0
Sequence length16
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCGAAGTAAAGGG17362810.7220969422280524No Hit
ACGTTACGTACCGTTG15466750.6432422448443386No Hit
TTTGCTGAGAAACCGT13189800.5485468221215095No Hit
CGAGTTTGTCGCATTG11611170.4828936302758653No Hit
TTACTGAGTTAAAGCG11297430.46984558709307234No Hit
ACATGCCTCAATCCAT11093250.46135400343442934No Hit
AGGTTTGCATTAGCGA10522300.4376089270807108No Hit
CGTAATAAGCTATCGC9223660.38360015931471914No Hit
CTCATAAGTGTTAACC9082330.37772242634147973No Hit
AACGGATTCAAACTAG7412950.30829505868516915No Hit
CCTTTATGTAGGTTTC7376270.3067695846495191No Hit
TAATCCGTCACAAAGC7304230.30377353368091964No Hit
CGTATTTAGCATCTAT7068490.2939694102031622No Hit
TGGACCAAGGCAATAC6605990.27473462990086817No Hit
GATCCTTCAACCGCCT6226480.25895129698730357No Hit
AGGCTTGAGGAACAAG5955150.24766703117233826No Hit
CCAAGGATCTAGCCAC4755890.19779134982027521No Hit
ACAAGTATCATGTTGT4278930.17795519671112456No Hit
TACCTGATCGCTTCTT4035630.16783666255426372No Hit
CCGCCTAAGATTCAAG4016470.16703982279082163No Hit
ACTGAATCAAGGCTTA3714870.15449666659801756No Hit
GTTACCGGTTTGCTGG3625590.15078362619717686No Hit
GTAGGAGCACATGCAA3202220.13317621228024232No Hit
GCTAATGCAACAGGCT3169260.13180544826129398No Hit
ACCTCATCACGGATTA2942470.1223735437753323No Hit
TGCATTAGTTTCCAGG2823380.11742074380516972No Hit
GGTGCATCACATAACG2820070.11728308516127654No Hit
TACCCGGTCCTAAATC2594260.10789193761519865No Hit
GGAGTCGGTGCACAAA2531920.10529929717402026No Hit
CAGTAAGGTAAGGTGC2459460.10228577894547057No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTA1151.8189894E-1210.0445861
CGACTTG245200.010.0322981
TCTGGGC301250.010.031251
CTGCTAA300600.010.0262091
GTGGGAA280400.010.0248841
GATTGGC238950.010.0235681
TGACTAT155250.010.0219431
ATGCGAT273850.010.0207451
ATTACGA238750.010.0172411
CGCTGGA106200.010.0162121
GAACGAT229000.010.0160771
ACTGAAT490050.010.0128171
ATGGCGG186300.010.0122381
CCTGGAC325350.010.0121711
CGATGAA101650.010.0100021
GCTGACT142300.010.0092941
AACGGAT811100.010.0092941
GACTGAC83250.010.0083911
GACTGCG64950.010.0059251
ATGGGCG139650.010.0050271
Produced by FastQC (version 0.11.5)