FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005761656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235763225
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT575550.032.87460344
TATGCCG721900.026.7981743
CGCGTAT328000.021.11635439
TCGCGTA333750.020.58051938
CGTATGC924650.020.30563441
ATCGCGT369300.019.3134937
TCGTATG640200.019.01733640
CTCGTAT658900.018.78617139
ACGAGAC1183150.018.63741922
TCTCGTA672550.018.40432238
GCGTATG382550.018.07516740
GTATGCC1100500.017.73881342
ATCTCGT722800.017.61113737
CACGAGA1284400.017.25386221
CCCACGA1324200.016.77513519
CCACGAG1351700.016.45177320
TATCGCG127650.016.25241736
TCCGAGC1403450.016.13353513
AGACGAT365550.015.35139525
TCTCCGA1472100.015.3109311
Produced by FastQC (version 0.11.5)