FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005761661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240449848
Sequences flagged as poor quality0
Sequence length49
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11739510.48823112585207373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG513150.011.1422355
GTATAGG287550.09.8045291
TCTATAC363300.09.7113163
GTATAGA398700.09.6224581
TAGACAG674350.09.5437625
GTGTTAG549250.09.5338071
TACACTG637450.09.4924065
CTATACT444400.09.4920194
CCTATAC292450.09.4762423
CTAATAC410600.09.2994853
TAGACTG482100.09.2193785
GTATTAG399300.09.1825321
GTCCTAG491550.09.1260751
CTAGGAC503000.09.0444583
GTCTAGA422750.08.749481
CTAGTAC202400.08.69977953
TAGGACA482350.08.682814
TCTGTCG294800.08.679868
GTCTAAG354300.08.6553731
GTACTAG215050.08.6199591
Produced by FastQC (version 0.11.5)