FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005761666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243293573
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT759000.034.19891744
TATGCCG960000.027.40520743
CGCGTAT433850.021.345139
CGTATGC1232000.021.24735341
TCGCGTA440300.020.9867238
TCGTATG847600.020.29749940
CTCGTAT860800.020.21776239
TCTCGTA877200.019.86919238
ATCGCGT481200.019.72797437
ACGAGAC1458850.019.46724322
GCGTATG484450.019.03248240
ATCTCGT935650.019.01521737
GTATGCC1397600.018.92983442
GCGTATC459200.018.40735433
CACGAGA1582250.017.98096821
CCCACGA1604050.017.78457619
TGCGTAT478300.017.7491732
CCACGAG1646200.017.3345820
TATCGCG188850.017.26467736
CTGCGTA501350.017.04283531
Produced by FastQC (version 0.11.5)