FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005761911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335305773
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTAT578487801.7344370782522556TruSeq Adapter, Index 15 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTATGCCGT47304490.14182954493390013TruSeq Adapter, Index 15 (96% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTATGCC33471300.10035451703096368TruSeq Adapter, Index 15 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)