FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005761923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510810080
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTAT1178124014.69220678769937TruSeq Adapter, Index 23 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTATGCCGT559721632.229247183841161TruSeq Adapter, Index 23 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTATGCC107846950.42953049638863966TruSeq Adapter, Index 23 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTATG71574970.28506724013151963TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCGCGTAT25460800.1014047227339473TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)