FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3238255
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA101550.035.747399
CGAGTAG14600.034.074853
CCGAGTA15150.033.8841552
GTAGCTG50800.030.8469876
AGTGCTG70150.030.4498796
TAGCTGG56150.029.7797187
GTGCTGG72450.029.2424627
GAGTAGC31450.029.1393994
TGCTGGG81300.029.0877198
AAGTGCT72250.028.7504255
AGTAGCT51900.028.5881945
AAAGTGC72850.028.3942724
CCCAAAG84500.028.3217951
CCCGAGT20100.027.7094971
CCAAAGT78850.027.4760912
CAAAGTG80050.026.8216633
GGAGGCT76650.026.6400269
TACTCGG15150.026.5006873
CTACTCG16300.025.6218762
GCTACTC35850.025.2457871
Produced by FastQC (version 0.11.5)