FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6414347
Sequences flagged as poor quality0
Sequence length100-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG3800.069.462091
TACTCGG27200.058.33863
CTACTCG28200.056.0732462
CGAGTAG20250.055.0005343
CGCGACT1700.052.9323461
CCGAGTA22150.052.460072
CTCGGGA32650.048.78215
TCGGGAG34650.047.5212826
GCTACTC73450.046.8812181
GCTGGGA205300.046.8077439
ACTCGGG34700.046.5896644
GAGTAGC48600.045.3384674
CCCGAGT27450.044.582721
GGAGGCT158950.043.5440569
GTAGCTG87150.040.8110476
AGTAGCT88250.040.6419145
GTGCTGG152850.039.4080057
TGCTGGG164600.038.775648
CCCAAAG175050.038.4854351
TAGCTGG96700.038.327997
Produced by FastQC (version 0.11.5)