FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5887145
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19550.040.373733
CTACTCG27550.039.936652
TACTCGG27950.039.1508333
GCTGGGA198900.038.1674429
CCGAGTA20450.038.0124132
GAGTAGC50850.035.5130274
GTAGCTG86850.034.7602276
GCTACTC67000.033.5673941
AGTAGCT91200.033.3715365
CCCAAAG148650.033.0758861
TCGGGAG35400.032.5923046
CTCGGGA34500.032.5830735
GTGCTGG141250.031.7427757
AAAGTGC135300.031.6878724
TAGCTGG102700.031.4919057
ACTCGGG35250.031.3822694
AGTGCTG139850.031.20486
GGAGGCT151250.031.1030869
CCAAAGT148550.030.995132
AAGTGCT141650.030.7303895
Produced by FastQC (version 0.11.5)