FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6173103
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG3400.056.383771
CGCGACT1151.3442332E-841.6749571
CCGAGTA20500.038.2195472
GCTGGGA194450.037.4211439
CGTCGCA1304.0272425E-836.7982185
CGAGTAG20700.035.8222273
TACTCGG24550.035.563353
GAGTAGC52450.034.7723854
CTACTCG24350.033.4047362
GTAGCTG88050.032.801826
AGTAGCT91800.031.3967365
TCGGGAG31250.030.9988166
GCTACTC60550.030.9680041
TAGCTGG101200.030.9030487
GTGCTGG135450.030.7704567
CCCAAAG157100.030.697481
CCCGAGT28800.030.5779151
AGTGCTG139250.029.930766
CCAAAGT149050.029.8143232
TGCTGGG155350.029.7898048
Produced by FastQC (version 0.11.5)