FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6173103
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19800.043.190363
CCGAGTA21150.041.848312
TACTCGG28250.041.702663
CTACTCG29050.041.378672
GCTGGGA191650.036.9611979
GAGTAGC49800.036.8644264
CCCGAGT27900.036.240331
GCTACTC65950.034.8358231
CCCAAAG153900.032.927171
CTCGGGA35050.032.75735
ACTCGGG37100.032.0758064
TCGGGAG38300.031.3719026
GTAGCTG87100.031.227476
GTGCTGG134800.031.1759387
AGTAGCT87550.030.872885
GGAGGCT158100.030.7015789
CCAAAGT150200.030.5786172
TGCTGGG150700.030.5434118
AGTGCTG137300.030.1286136
CGTCGAG1602.4833025E-729.9143521
Produced by FastQC (version 0.11.5)