FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6427020
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG4350.059.689371
CCGAGTA23250.051.3380622
CGAGTAG21900.050.363953
TACTCGG27700.046.563623
GCTGGGA205700.046.317619
CTACTCG28500.045.2653852
GAGTAGC52000.043.811124
CCCGAGT29150.043.293781
GCTACTC69500.043.1806681
GGAGGCT159050.040.579289
GTAGCTG91100.039.9589846
GTGCTGG148650.039.4497077
TCGGGAG36500.039.133746
CTCGGGA36100.039.0668075
AGTAGCT93350.038.93165
CGCGACT1252.6160706E-838.6453931
TGCTGGG163350.038.293658
AGTGCTG155800.037.677966
TAGCTGG103200.037.2594877
CCCAAAG173200.036.7460371
Produced by FastQC (version 0.11.5)