FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4974207
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG20700.035.619532
CGAGTAG17100.035.404723
TACTCGG22150.034.3689233
CCGAGTA18050.033.210562
GCTGGGA157100.032.799749
CCCAAAG119500.031.4119781
GAGTAGC41450.029.6453464
CTCGGGA27400.028.8763185
AAAGTGC108850.028.6903924
CCAAAGT118000.028.6517052
CCCGAGT22900.028.279851
AGTGCTG111750.028.0954676
TCGGGAG29200.027.9067046
GCTACTC49900.027.878941
CAAAGTG119450.027.6503963
GGAGGCT125400.027.4737829
GTGCTGG113700.027.2452837
AAGTGCT114550.026.948255
ACTCGGG28200.026.7823454
GTAGCTG73400.026.775056
Produced by FastQC (version 0.11.5)