FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5319635
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG1202.7084732E-547.84845120-124
CGCGACT1052.9830335E-740.128721
CGTCGAG2400.037.6206741
CGAGTAG19000.034.7811433
CCGAGTA19850.034.2084122
GCTGGGA173600.033.9792259
TACTCGG20100.032.279923
CCCAAAG133900.031.917171
CCCGAGT26050.030.9630451
CTACTCG21150.030.685162
GCTACTC49300.029.79131
AGTGCTG121200.029.7892996
GTGCTGG121350.029.6039627
CCAAAGT130550.029.5050352
GAGTAGC46700.029.2001554
AAGTGCT123250.029.0985765
AAAGTGC118450.028.7054
TGCTGGG134800.028.252128
GTAGCTG77650.028.1610166
GGAGGCT121450.027.9449049
Produced by FastQC (version 0.11.5)