FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4856806
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG20200.044.442223
CCGAGTA21300.043.843352
TACTCGG22800.043.3116723
CTACTCG22300.042.1457252
CGTCGAG3000.039.9654921
GAGTAGC46800.038.6192254
GCTGGGA180700.038.479369
CCCGAGT26300.037.6101071
GCTACTC52300.036.4503631
GTAGCTG80750.036.1679766
TCGGGAG31550.035.8516656
TAGCTGG89200.035.3584067
AGTAGCT82150.034.8234445
GGAGGCT133000.034.2833449
CTCGGGA32000.034.2257165
CCCAAAG136450.034.049021
ACTCGGG32000.034.0379874
CGCGAAC902.3101304E-433.257242
CCAAAGT130700.033.1147462
AAAGTGC121050.031.5426644
Produced by FastQC (version 0.11.5)