FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4856806
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG25350.047.2185633
CTACTCG25400.046.1835752
CGAGTAG18850.046.0380973
CCGAGTA20450.045.0705262
GAGTAGC46550.042.6858184
CCCGAGT25300.040.7066651
GCTACTC56600.039.98831
GCTGGGA182300.039.812429
GTAGCTG80950.037.622696
TCGGGAG34700.037.5903136
ACTCGGG33000.037.3614
AGTAGCT80150.037.26095
CTCGGGA34050.036.7354555
GGAGGCT146100.035.0155039
CCCAAAG133600.033.523761
TAGCTGG96800.033.194477
CCAAAGT128650.032.286052
AAAGTGC117000.032.0735364
AGTGCTG125000.031.3530796
AAGTGCT122300.031.2702035
Produced by FastQC (version 0.11.5)