FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4550326
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2950.062.940531
CCGAGTA19050.040.8085372
CGAGTAG19000.039.649133
TACTCGG18450.037.266473
CTACTCG17900.037.082812
GAGTAGC43100.036.7604334
GCTGGGA162400.035.81449
GTAGCTG73650.034.9874046
TCGGGAG24350.034.3744936
TCGCGTT1051.4364152E-534.1644135
CTCGGGA23000.034.0530055
CCCGAGT23750.033.289021
AGTAGCT76100.032.368655
TAGCTGG85650.031.5510257
CGCGAAC1152.6722755E-531.2001082
CCCAAAG120300.029.9226131
GGAGGCT110950.029.7939919
AAAGTGC104850.029.252364
GCTACTC45100.029.2170811
ACTCGGG25900.029.0859184
Produced by FastQC (version 0.11.5)