FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4550326
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16700.037.9521753
GCTGGGA151600.034.9711079
CCGAGTA18450.034.67762
GAGTAGC41700.032.5482444
GTAGCTG67850.031.9786
CCCAAAG116250.030.9751341
AGTGCTG100750.030.6125286
GTGCTGG100800.030.3601537
AAAGTGC99550.030.271024
AAGTGCT102450.029.9390755
GCTACTC49400.029.907471
GGAGGCT118550.029.8473059
AGTAGCT72450.029.6270925
TACTCGG20450.029.530813
CCAAAGT110300.029.4364832
CTACTCG20350.029.3830742
TAGCTGG82350.029.0329347
CCCGAGT23500.029.016611
CAAAGTG109950.028.8766353
TGCTGGG117050.028.1869378
Produced by FastQC (version 0.11.5)