FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4807390
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2750.067.67891
TACTCGG21300.065.555533
CTACTCG22050.061.7078742
CGAGTAG17000.059.575923
CCGAGTA18250.056.8206942
GCTACTC54150.054.5495221
TCGGGAG29450.052.49776
GCTGGGA165900.052.1516349
CCCGAGT22650.050.0977171
GGAGGCT127400.049.711029
ACTCGGG29500.048.955944
CTCGGGA29750.047.7388275
GAGTAGC42400.047.3466574
GTGCTGG124850.046.7494287
CCCAAAG142150.046.4590341
TGCTGGG137300.045.210198
GTAGCTG67650.044.4674036
AGTGCTG131900.044.023536
AAAGTGC129350.043.9189834
CCAAAGT143050.042.5725022
Produced by FastQC (version 0.11.5)