FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4807390
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG27200.069.403023
CTACTCG27150.069.090812
GCTACTC65200.060.8614161
TCGGGAG33850.058.753116
CGAGTAG17250.058.7130053
CTCGGGA33450.056.0741045
GCTGGGA167700.055.687799
ACTCGGG34550.054.9848944
CCGAGTA19400.054.678592
GGAGGCT144350.053.947779
CCCGAGT21500.050.462851
TGCTGGG134250.048.7705158
GAGTAGC40550.048.1791274
GTGCTGG125750.047.6840367
CCCAAAG143100.047.4174771
AGTGCTG131600.045.4737786
GTAGCTG70750.045.043976
AAAGTGC129600.044.391364
AGTAGCT72800.042.9688035
AAGTGCT134250.042.9412735
Produced by FastQC (version 0.11.5)