FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6213560
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG3850.065.391151
CGAGTAG24550.040.7065473
CCGAGTA25950.039.9014242
CTACTCG24750.037.9668122
TACTCGG25300.037.844153
GAGTAGC59300.035.0157624
CCCGAGT31600.034.1441151
GCTGGGA222100.033.9983379
GCTACTC63300.033.237921
AGTAGCT103750.032.8762665
GTAGCTG107000.031.8220216
CCCAAAG163950.030.820991
TAGCTGG117700.030.606657
ACTCGGG32350.029.226154
CCAAAGT160050.028.570472
GGAGGCT154050.028.5089519
GTCGAGA9250.028.4702072
AGTGCTG149350.028.2477056
GTGCTGG150300.028.029127
AAAGTGC148700.027.8875964
Produced by FastQC (version 0.11.5)