FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6213560
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA25250.037.683162
GCTGGGA218500.036.085469
TACTCGG29850.035.684843
CGAGTAG24750.035.30093
CTACTCG29800.034.740932
CCCGAGT30500.034.349281
GAGTAGC58700.032.5204964
GTAGCTG97150.032.390026
AGTAGCT98650.031.966915
CCCAAAG164050.031.638971
GCTACTC73150.031.0173241
CCAAAGT156750.030.541762
TTGGGAG127350.030.3460256
AAAGTGC142400.030.2991094
GGAGGCT171400.030.0511139
AGTGCTG147850.030.0227766
TAGCTGG113200.029.9645947
GTGCTGG153100.029.4623857
AAGTGCT150000.029.201625
TCGCGTT2052.2864697E-929.1899435
Produced by FastQC (version 0.11.5)