FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4807848
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19650.061.980022
TACTCGG26850.060.764233
CGAGTAG19050.060.454633
CTACTCG26200.060.224432
GCTACTC61750.051.664861
GCTGGGA189100.051.377699
CCCGAGT24650.051.184691
GAGTAGC47100.049.9205674
TCGGGAG34150.049.88236
ACTCGGG33250.049.788814
CTCGGGA33400.049.206555
CGCGACT1152.5102054E-1047.0199741
GGAGGCT146300.046.73179
GTAGCTG79550.046.296386
GTGCTGG135700.045.4830787
AGTAGCT82500.045.2952775
TGCTGGG144450.045.1712888
CCCAAAG156100.044.4546051
AAAGTGC135350.044.404174
CGTCGAG2450.044.1411931
Produced by FastQC (version 0.11.5)