FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4807848
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG30750.063.9799463
CTACTCG31000.061.723232
GCTACTC68950.058.0444031
TCGGGAG36450.057.412046
CTCGGGA35650.057.367355
ACTCGGG37300.054.0296334
GGAGGCT156000.053.2331479
GCTGGGA189600.052.8755539
CCGAGTA18850.051.8675732
CGAGTAG17600.051.4611433
CCCAAAG152900.048.4257661
GTGCTGG139150.047.057417
GAGTAGC44750.046.9112054
AGTGCTG139200.046.9098176
AAAGTGC134600.046.4772954
GTAGCTG76550.046.3715486
TGCTGGG146800.046.2366528
AAGTGCT140650.045.4982455
CCCGAGT22750.045.364471
AGTAGCT78550.044.742625
Produced by FastQC (version 0.11.5)