FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525917
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG17600.072.249962
TACTCGG18400.070.734383
CGAGTAG12000.068.474353
GCTACTC40900.065.6411
CCGAGTA13100.063.18622
GAGTAGC29150.062.3424844
GCTGGGA127600.061.5647169
CGTCGAG1200.060.0607761
CCCGAGT13400.059.612561
TCGGGAG23000.058.411756
GTAGCTG49750.058.2282946
ACTCGGG22200.058.0852054
CTCGGGA22800.057.6088685
GGAGGCT94950.057.4090239
AGTAGCT51450.056.7706225
AAAGTGC87150.055.7439424
TAGCTGG54550.055.5223737
CCCAAAG94300.055.347631
CCAAAGT91650.055.105392
AGTGCTG90900.054.433986
Produced by FastQC (version 0.11.5)