FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525917
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG21900.076.415582
TACTCGG22850.073.760653
GCTACTC50100.070.179581
CGAGTAG12600.068.072253
GCTGGGA130800.065.5404369
GAGTAGC29800.064.810334
CCGAGTA13400.064.010772
GTAGCTG49450.062.1953436
TCGGGAG28600.062.048676
CTCGGGA27400.061.7273145
ACTCGGG26700.061.3276024
AGTAGCT49300.061.1927385
CCCAAAG101300.060.1776351
AAAGTGC89850.060.0131234
GGAGGCT111300.059.8950089
CCCGAGT14750.058.9831
CCAAAGT98000.058.6355862
AAGTGCT93800.057.8022045
CTACTTG31700.057.522292
GTGCTGG95600.057.0038537
Produced by FastQC (version 0.11.5)