FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8886751
Sequences flagged as poor quality0
Sequence length100-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG5550.074.3214651
CGCGACT2250.055.7949561
CGAGTAG25550.050.4513173
CCGAGTA28400.046.868052
GCTGGGA272100.044.5170029
TACTCGG36050.043.3716283
CTACTCG35650.042.8618352
GAGTAGC69200.041.997914
CCCGAGT35000.040.6506081
GCTACTC89550.039.9870531
GGAGGCT200400.039.6611149
GTGCTGG194800.038.2325177
GTAGCTG120250.038.11396
TAGCTGG129300.037.800077
CCCAAAG229200.037.088831
CTCGGGA46600.037.010015
TCGGGAG49750.036.705886
AGTAGCT122600.036.5558365
TGCTGGG217200.036.5386628
AGTGCTG204250.035.9084856
Produced by FastQC (version 0.11.5)