FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6136447
Sequences flagged as poor quality0
Sequence length100-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG3500.075.275021
CGCGACT901.544322E-953.224761
CGAGTAG16550.046.5892453
GCTGGGA170350.044.9393359
TACTCGG23650.044.228363
CCGAGTA18050.044.0500372
CTACTCG23350.042.50042
CGTTGCG1750.041.05911
GCTACTC54150.040.1397251
GAGTAGC42700.039.0531544
CCCAAAG148450.038.8026241
CCCGAGT23800.038.744491
GTGCTGG131200.037.5387847
GGAGGCT131800.037.4074559
TGCTGGG139400.036.9113588
CTCGGGA29500.036.267825
AGTGCTG134150.036.2234886
TCGGGAG31850.035.46856
AGTAGCT77250.035.282295
GTAGCTG76850.035.0770536
Produced by FastQC (version 0.11.5)