FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6228634
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2950.042.6384621
TACTCGG23650.038.934663
CCGAGTA24500.038.3227922
CGAGTAG23900.037.5266843
CTACTCG23600.037.2502252
GCTGGGA201800.035.2794959
CCCGAGT31100.034.0892221
GAGTAGC55800.031.716624
CCCAAAG159850.030.6510181
GCTACTC60100.030.4965841
GTAGCTG95850.030.4408766
TCGGGAG34600.030.413476
AGTAGCT97450.029.757255
TAGCTGG103700.029.7509167
GGAGGCT146250.029.5102659
CTCGGGA33850.029.321245
ACTCGGG34400.028.67844
AAAGTGC140650.028.6516464
CCAAAGT155200.028.3987582
AGTGCTG142750.028.313926
Produced by FastQC (version 0.11.5)