FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6228634
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21400.040.5071333
TACTCGG28050.039.6421663
CTACTCG28500.038.5976332
GCTGGGA210100.038.5450369
CCGAGTA22350.037.181342
GAGTAGC54400.035.8260964
GTAGCTG93100.033.6382456
GCTACTC67350.033.5656781
AGTAGCT96300.032.901295
TAGCTGG103650.032.8683747
CCCAAAG163450.032.162161
GTGCTGG147800.031.9062087
CCCGAGT29350.031.78761
CTCGGGA36850.030.985555
GGAGGCT156050.030.9458779
TGCTGGG163400.030.542438
AAAGTGC143550.030.485174
ACTCGGG37200.030.3738334
AGTGCTG148750.030.3348396
TCGGGAG38300.029.9608526
Produced by FastQC (version 0.11.5)