FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6342938
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG32350.063.0751273
CTACTCG32700.062.5943572
CGAGTAG23350.060.2225383
CCGAGTA24700.060.0912932
GCTACTC76150.057.306031
GCTGGGA234400.054.674119
CCCGAGT28850.053.8136941
CTCGGGA40350.052.6452835
GAGTAGC58850.052.566844
TCGGGAG41950.051.6354336
ACTCGGG40650.050.7839364
GGAGGCT179650.049.6225289
CGTCGAG3050.049.1336441
GTAGCTG98250.048.78336
GTGCTGG168100.048.446037
TGCTGGG184750.047.248798
CCCAAAG195900.047.1221541
AGTAGCT102750.046.647175
AGTGCTG178500.046.294216
AAAGTGC175600.046.1724134
Produced by FastQC (version 0.11.5)