FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4648568
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG20550.055.2968373
CTACTCG21100.053.8635642
CCGAGTA17850.052.612072
CGAGTAG17250.051.660153
CGTCGAG2650.049.730481
CCCGAGT21950.047.7583541
GCTACTC51950.047.7376061
TCGGGAG26900.046.912016
CTCGGGA25650.046.3996665
GCTGGGA160350.044.7527129
ACTCGGG27150.042.95414
GAGTAGC40900.042.258534
GGAGGCT120500.042.1831479
CCCAAAG128500.041.116041
GTGCTGG112150.039.7821927
AGTAGCT70150.039.387965
GTAGCTG69550.038.9542546
AGTGCTG117750.038.804876
TGCTGGG124200.038.2301528
AAAGTGC117350.037.2541054
Produced by FastQC (version 0.11.5)