FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6144793
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA22550.052.082822
TACTCGG33700.051.0300943
CGAGTAG21850.049.9107743
CTACTCG31900.049.2147832
GCTGGGA225500.047.278539
GCTACTC80700.045.0841521
CCCGAGT29500.044.090651
AGTAGCT93750.043.0773355
GGAGGCT185200.042.9808049
CTCGGGA41800.042.57365
TCGGGAG45100.042.3654446
GTAGCTG96050.041.9053546
CCCAAAG177550.041.692221
ACTCGGG43050.041.199594
GAGTAGC55450.041.1716044
AAAGTGC159500.040.8360024
GTGCTGG163200.040.077457
AGTGCTG164100.039.492656
AAGTGCT165650.039.3547975
TGCTGGG177350.039.277718
Produced by FastQC (version 0.11.5)