FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5150001
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG3400.049.3598371
CGAGTAG20700.042.486623
CCGAGTA21200.042.343132
GCTGGGA182350.037.2985959
GAGTAGC47900.037.22014
CCCGAGT26400.036.3254171
TACTCGG21050.035.243163
CTACTCG20500.035.0311972
GTAGCTG83550.032.8678636
CCCAAAG134950.032.55561
GCTACTC52300.032.547021
TCGCGTT1501.4101533E-731.9086325
AGTAGCT84300.031.8660535
GGAGGCT127900.030.774659
GTGCTGG125350.030.641627
TAGCTGG92750.030.5749287
AAAGTGC122850.030.0960944
AGTGCTG127900.029.9842196
CCAAAGT134350.029.7999822
ACGTCGA2052.248271E-929.2375341
Produced by FastQC (version 0.11.5)