FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5150001
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18300.045.1141553
CCGAGTA19450.043.0644152
TACTCGG24500.041.267243
GCTGGGA178900.040.147389
GAGTAGC45600.039.2271654
CTACTCG25500.038.947362
GTAGCTG80200.036.8363956
GCTACTC58400.035.3540151
AGTAGCT81600.034.8970685
CCCGAGT25900.034.89071
TAGCTGG91150.034.380147
GGAGGCT139400.034.234559
CCCAAAG133750.034.1399771
AGTGCTG124950.033.120316
GTGCTGG125150.032.972457
CCAAAGT130300.032.8300632
TCGGGAG32800.032.6365136
CTCGGGA31150.032.4564365
ACTCGGG31900.032.4441224
AAAGTGC119500.032.290324
Produced by FastQC (version 0.11.5)