FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5346527
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2850.063.1526031
CTACTCG25500.059.436432
TACTCGG26400.059.2150043
CGAGTAG17600.056.8327943
CCGAGTA19450.054.516262
GCTACTC59350.054.3846631
GCTGGGA186800.051.353159
TCGGGAG31750.050.7443246
GGAGGCT141250.048.455079
GAGTAGC47050.048.3720864
CTCGGGA32200.048.3606725
CCCGAGT23200.047.840821
ACTCGGG34400.046.4861534
GTAGCTG80600.045.6259846
AGTAGCT81250.044.5971265
CCCAAAG156350.043.7443351
TAGCTGG88750.043.7302067
AGTGCTG136700.043.0689166
GTGCTGG135700.043.0328037
TGCTGGG147300.042.3184178
Produced by FastQC (version 0.11.5)