FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5346527
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG29700.064.120773
CTACTCG30600.062.6286242
CGAGTAG18750.059.407293
CCGAGTA19800.057.7713552
GCTACTC69400.057.321231
CTCGGGA35750.055.276125
TCGGGAG36850.054.5868036
GCTGGGA196500.053.438839
ACTCGGG37200.052.8030474
GGAGGCT157050.052.3005339
CCCGAGT25150.049.3104821
CCCAAAG161450.047.943681
GTGCTGG145600.046.9992647
AAAGTGC141450.046.8676034
GAGTAGC47000.046.762354
TGCTGGG155900.046.3523148
AGTGCTG147600.045.914096
GTAGCTG78750.045.30876
AAGTGCT150250.044.9566845
CCAAAGT160600.044.226592
Produced by FastQC (version 0.11.5)