FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6568247
Sequences flagged as poor quality0
Sequence length100-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG3850.066.2087251
CTACTCG27050.058.924542
TACTCGG26750.058.667523
CGAGTAG19550.055.7888643
CCGAGTA20100.055.479112
GCTACTC69000.051.719571
GCTGGGA199150.050.1632929
CTCGGGA33950.048.723455
CCCGAGT25300.048.4571041
GGAGGCT153200.048.046669
CGCGACT1301.4551915E-1146.6856351
TCGGGAG35500.046.254376
GAGTAGC47850.044.1935234
ACTCGGG37250.044.0816844
GTGCTGG153100.043.019587
TGCTGGG165200.042.4296078
CCCAAAG181000.041.7797781
AGTGCTG162950.040.902536
GTAGCTG85000.040.0616876
AAAGTGC163400.039.937314
Produced by FastQC (version 0.11.5)