FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6568247
Sequences flagged as poor quality0
Sequence length100-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG31450.062.422843
CTACTCG31800.061.7386172
GCTACTC78750.057.807021
CGAGTAG20150.057.735823
CCGAGTA21650.054.5777632
GCTGGGA213650.053.0729569
CTCGGGA38250.052.4315345
GGAGGCT176600.051.3453879
TCGGGAG41050.050.0234226
ACTCGGG40850.049.9866144
CCCGAGT26400.049.3657461
GAGTAGC49900.048.206524
CCCAAAG185650.046.886811
GTGCTGG160900.046.1169177
GTAGCTG86050.045.967326
TGCTGGG176250.045.6053128
AGTGCTG166150.044.8062326
AAAGTGC165900.044.5218474
TAGCTGG99550.042.7754257
AGTAGCT93050.042.4548385
Produced by FastQC (version 0.11.5)