FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6434789
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2550.060.7122151
CTACTCG28850.058.732672
TACTCGG28950.057.900193
CGAGTAG22000.054.846993
GCTACTC73450.053.180811
CCGAGTA24300.051.6246222
GCTGGGA216350.050.872049
TCGGGAG36850.048.712316
GGAGGCT167600.047.939979
CTCGGGA36450.047.779265
CCCGAGT28850.047.057841
GAGTAGC54200.046.7167744
ACTCGGG37450.045.391734
CCCAAAG187050.044.7261541
GTGCTGG161400.043.750087
TGCTGGG171400.043.444558
GTAGCTG92050.043.0050776
AGTAGCT93400.042.956235
AAAGTGC165700.042.435064
AGTGCTG167750.042.3777586
Produced by FastQC (version 0.11.5)