FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6434789
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG34250.061.7827073
CTACTCG33100.060.5191652
CGAGTAG22300.057.040913
GCTACTC81850.055.3625951
CCGAGTA23400.054.8692362
GCTGGGA227650.052.7763259
TCGGGAG41650.052.642566
CTCGGGA41750.050.679295
GGAGGCT187000.050.013679
GAGTAGC53850.048.566614
CCCGAGT29400.048.342681
ACTCGGG45150.047.3931434
CCCAAAG192800.046.8214531
GTGCTGG166750.046.502587
AGTAGCT93000.046.205365
AGTGCTG169550.046.0843626
GTAGCTG91800.045.4380876
AAAGTGC167400.045.3780864
AAGTGCT173300.045.0987175
TGCTGGG181750.045.0832758
Produced by FastQC (version 0.11.5)