FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883935
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2050.052.6029551
CGAGTAG10000.049.6312833
CCGAGTA11650.046.2061122
TACTCGG12850.040.9502833
CCCGAGT14600.040.2128371
CTACTCG12950.040.182072
GCTGGGA103900.038.842349
GAGTAGC27850.038.0016864
CGCGACT957.1329705E-637.8372151
GCTACTC28150.037.66921
AGTAGCT46550.035.8382535
GTAGCTG47200.034.964676
TCGGGAG18300.033.981666
GGAGGCT73750.033.9679379
CCCAAAG80500.033.415041
TAGCTGG52750.033.326317
CGAGACT14950.033.260471
CTCGGGA18350.032.585645
ACTCGGG17900.031.7340414
AAAGTGC70550.031.5283134
Produced by FastQC (version 0.11.5)