FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883935
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10600.041.18053
CCGAGTA11600.040.723372
CTACTCG13600.039.571112
GCTGGGA106150.039.139159
CCCGAGT14150.038.888981
TACTCGG13700.038.84583
GCTACTC31750.036.3587341
CCCAAAG78150.035.7423481
GACCGAT1703.110472E-1035.1639489
GGAGGCT82000.034.8466159
AGTGCTG72250.034.004436
CCAAAGT75700.033.7292442
CGGGTTC9400.033.7050179
GAGTAGC27000.033.662544
AAGTGCT70550.033.647525
GTGCTGG73250.033.622397
AAAGTGC68750.032.963524
GTAGCTG46700.032.896356
AGTAGCT46900.032.383255
CAAAGTG73850.032.226063
Produced by FastQC (version 0.11.5)