FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085485
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG13950.033.872253
CTACTCG13750.032.624942
CGAGTAG9550.032.5683
GCTGGGA103400.032.032469
CCGAGTA10800.031.013832
TCGGGAG17650.029.4711276
GAGTAGC23700.029.0228884
CCCAAAG75950.028.5175881
GCTACTC32600.028.447631
AGTAGCT42550.028.1134765
GTGCTGG72750.027.9426827
AGTGCTG72750.027.6139456
CCCGAGT13500.027.0351071
AAAGTGC68200.026.748944
CTCGGGA18200.026.6193685
TGCTGGG80900.026.6049278
ACTCGGG19800.026.5832844
GTAGCTG45150.026.2198146
CCAAAGT76450.026.0530092
TAGCTGG52300.025.9505377
Produced by FastQC (version 0.11.5)