FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5649031
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG5350.053.6528631
CCGAGTA23500.047.2607042
CGAGTAG22250.047.223793
CTACTCG26500.042.5864072
TACTCGG28050.041.503043
CGCGACT1603.6379788E-1241.11291
GCTACTC61750.039.608811
CCCGAGT30350.039.604341
GAGTAGC52550.039.5331654
GCTGGGA215050.039.163479
GTAGCTG90250.036.8436856
AGTAGCT90750.036.6410185
GGAGGCT154400.036.1458059
TCGGGAG34100.035.888446
CCCAAAG160000.035.1328431
TAGCTGG100650.034.8157357
CTCGGGA34300.033.590945
AAAGTGC138500.033.318454
CCAAAGT154850.033.2006842
ACTCGGG36800.033.09314
Produced by FastQC (version 0.11.5)