FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5649031
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21400.050.7695243
CCGAGTA22950.048.1222652
CTACTCG29100.047.593982
TACTCGG29400.047.5131723
GCTACTC69600.043.2427141
GCTGGGA212150.042.6973729
GAGTAGC52250.041.129554
CCCGAGT29050.040.084841
GTAGCTG88850.039.9634256
AGTAGCT90800.038.2616235
TCGGGAG39050.038.052356
GGAGGCT164000.037.4043169
ACTCGGG39450.037.0729264
CCCAAAG164400.036.8685341
CTCGGGA38400.036.6865655
TAGCTGG105100.036.453197
AAAGTGC141050.036.1851844
AGTGCTG148050.035.5922556
AAGTGCT149100.035.2715455
CCAAAGT157950.035.1495972
Produced by FastQC (version 0.11.5)