FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7378051
Sequences flagged as poor quality0
Sequence length100-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG4700.076.271081
TACTCGG24550.055.879463
CTACTCG27150.050.3170432
CGCGACT1207.2759576E-1249.7880631
CGAGTAG17750.049.3963473
CCGAGTA19250.049.273782
GCTGGGA189050.047.5712559
GCTACTC68200.046.517531
TACGCGG909.550968E-846.389494
CCCGAGT23500.046.2711221
GGAGGCT142050.045.426159
CTCGGGA31750.043.770575
TCGGGAG33850.042.4638676
GTGCTGG147750.041.860997
TGCTGGG155550.041.5989728
CGGAGGT35950.041.310337
CGCGAGT1455.638867E-1141.2039151
GAGTAGC47550.040.2641564
CCCAAAG182150.039.819521
ACTCGGG35400.039.5939374
Produced by FastQC (version 0.11.5)