FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124821
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGC18750.062.255664
CCGAGTA8350.061.9095042
TACTCGG9250.061.2941863
CGAGTAG8550.060.4613233
CTACTCG10000.058.3646852
GCTGGGA70450.055.388179
AGTAGCT32300.051.627325
GCTACTC20100.050.6098631
CCCGAGT11650.048.669221
GTAGCTG34350.047.089836
TAGCTGG38000.046.5162167
CTCGGGA12600.042.3507925
TCGGGAG13100.040.734356
CGGGTTC10450.039.8938379
ACTCGGG14350.039.5101934
CCCAAAG55900.039.080571
GGAGGCT52550.038.3967749
CCGGGTT11900.037.835458
CCAAAGT51750.037.0569422
CAAAGTG51900.035.664633
Produced by FastQC (version 0.11.5)