FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149889
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8600.0103.423563
GCTACTC23450.092.318541
GCTGGGA74600.090.429559
CTACTCG9050.089.009172
GAGTAGC19350.088.459074
GGAGGCT54300.087.7691049
CCGAGTA7700.087.178892
CGAGTAG7450.085.599143
AGTAGCT33100.084.6665045
TAGCTGG36250.080.086867
GTAGCTG36350.078.019916
AAAGTGC56200.074.948094
TCGGGAG13850.073.910016
ACTCGGG12600.073.25134
AAGTGCT58900.071.797355
CCAAAGT63000.071.65622
CTCGGGA12950.071.271535
AGTGCTG58900.070.657716
CCCAAAG69200.070.5714341
GTGCTGG60000.070.4810647
Produced by FastQC (version 0.11.5)