FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071641
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA7300.064.001142
CGAGTAG7850.059.516973
GCTGGGA65700.057.397849
GAGTAGC17850.056.0874254
TAGCTGG31900.051.2610977
AGTAGCT29650.050.6489335
CCCGAGT10950.050.288951
GTAGCTG31000.050.058036
CTACTCG7350.045.4041022
CCCAAAG51050.044.4545171
CCAAAGT47600.043.4677542
AGTGCTG47150.043.1748286
AAGTGCT47000.042.95765
AAAGTGC45950.040.6710134
CAAAGTG50500.040.641173
GTGCTGG50000.040.046427
TACTCGG7950.039.8785133
GCTACTC19400.039.5666121
TGCTGGG57700.037.015768
AGCTGGG51350.036.0690738
Produced by FastQC (version 0.11.5)